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Pfizer Senior Scientist Immunotherapeutic Design in La Jolla, California

ROLE SUMMARY

The Cancer Immunology Discovery (CID) group in La Jolla, CA is focused on the generation of large molecule immunotherapeutics that promote anti-tumor activity or counter immunosuppression in the tumor microenvironment. CID's efforts are supported by a world class antibody generation program, and a commitment to effective biotherapeutic design that stresses both ingenuity and simplicity. Our team takes a holistic approach to understanding the interplay of tumor, stromal, and immune cells in the tumor microenvironment, striving to define the axes of intercellular communication that could be leveraged to generate novel therapeutics.

We are seeking a qualified candidate to join us at a Senior Scientist level to contribute to our hybridoma generation and antibody characterization activities for the identification of novel biotherapeutics. The successful candidate will work closely with individuals in antibody production, protein engineering and immunology/oncology groups to innovate and develop the next-generation of protein therapeutics for cancer immunotherapy.

The successful candidate will demonstrate clear and professional verbal and written communication, maintain an electronic notebook, and present scientific results to collaborators and at team meetings. This position will not have any direct reports. There will be less than 5% travel.

ROLE RESPONSIBILITIES

  • Carry out hands-on lab work for the generation and screening of hybridomas and characterization of antibodies.

  • Cell-culture, rigorously maintain, cryopreserve and catalog cell lines.

  • Antibody screening (ELISA, FACS), preferably high-throughput.

  • Antibody expression, purification and analysis (affinity chromatography, size exclusion chromatography using FPLC, SDS-PAGE, western blot).

  • Flow cytometry and sorting

  • Implement robotic or high-throughput methods for antibody screening, purification, and sequencing.

  • Antibody sequencing and analysis.

  • Recombinant antibody generation and production

  • Comprehensive data analysis and reporting for effective selection of drug candidates.

BASIC QUALIFICATIONS

  • Recent PhD in Biochemistry, Molecular Biology, or related discipline or BS/MS with a minimum of 7 years of laboratory experience. Previous industrial experience is strongly preferred.

  • Hybridoma fusion

  • Antibody screening using ELISA and flow cytometry, preferably high throughput.

  • Cell culture, cryopreservation.

  • Antibody expression and purification

  • Experienced in flow cytometry and data analysis, cell sorting an asset

  • Strong understanding of antibody technology and protein biochemistry.

PREFERRED QUALIFICATIONS

  • Antibody discovery using antigen-specific B cell or hybridoma selection/isolation.

  • High-throughput screening of antibodies

  • Single cell sorting via FACS

  • Antibody sequencing (5'-RACE, RT-PCR, NGS)

  • Hands-on experience with instruments such as GE AKTA Pure/Avant, Perkin-Elmer LabChip, liquid handling robots, Biacore T200 or 8K.

  • Antibody affinity measurements (SPR or BLI)

  • Advanced molecular design proficiency.

  • Motivated, proactive, and eager to learn.

  • Excellent communication and presentation skills.

PHYSICAL/MENTAL REQUIREMENTS

NON-STANDARD WORK SCHEDULE, TRAVEL OR ENVIRONMENT REQUIREMENTS

  • May require weekend work, as needed.

  • There will be less than 5% travel.

*LI-PFE

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