Pfizer Senior Associate Scientist Mammalian Protein Expression and Molecular Biology (Non in La Jolla, California
BioMedicine Design (BMD) is seeking a highly motivated scientist to join a protein expression and molecular biology group supporting the Biotherapeutics portfolio of Pfizer, Inc. in La Jolla, CA. Responsibilities will include execution on multiple steps of protein expression for biotherapeutic leads, protein reagents, immunogens, and panning reagents, as well as the design and building of DNA constructs. This includes expertise in mammalian tissue culture techniques using various cell lines and cell culture technologies. The ideal candidate will be proficient in the design of optimal processes that are consistent with team material needs for quantity (milligram to gram scale) and purity.
Perform protein expression in mammalian transient and stable cell lines, from various shake flask scales to bioreactor systems
Perform and/or coordinate the generation of DNA constructs tailored for the expression of recombinant proteins
Design, assess and troubleshoot cell culture and expression protocols.
Deliver cell culture supernatants and data analysis packages to project teams in a timely manner.
Present results regularly at team meetings
Perform diligent and timely notekeeping and work process tracking
BA/BS in Biochemistry, Biology, or related field with 3+ years of R&D experience in an industry setting, or a Masters degree with 2+ years of industry experience
Experience in protein expression using transient and/or stable mammalian cell culture systems
Expertise in design, execution, and troubleshooting of expression processes.
Expertise in techniques for the monitoring of cell culture density, viability and protein titer
Experience with data analysis and presentation software
Strong written and oral communication skills
The ability to work closely with other group members within teams is essential
The individual should be scientifically creative and driven by a willingness to embrace innovation within drug discovery and an eagerness to challenge and meet aggressive timelines
- Expertise in the generation and design of plasmid constructs for expression of recombinant proteins, using cutting-edge molecular biology techniques
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Other Job Details
- Eligible for Employee Referral Bonus
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