Pfizer Principal Scientist in La Jolla, California
Pfizer is seeking applications for a Principal Scientist with extensive mass spectrometry-based proteomics experience. Such a candidate will be a highly motivated and creative scientist with the desire to lead and implement innovative proteomic capabilities for our oncology pipeline. This person will align with our Chemical Biology group enabling impact across the small molecule oncology portfolio from early exploratory target identification through the drug discovery pipeline and into early clinical development.
The individual will join the development and implementation of innovative mass spectrometry platforms. Their work will enable quantitative chemical proteomics methodologies for a deeper understanding of our molecules and cellular targets. Relevant methodologies include but are not limited to the identification of novel oncology targets, small molecule target deconvolution, small molecule target occupancy studies using chemical probes, direct substrate identification and characterization, protein turnover studies, protein post-translation modification studies and small molecule-protein interactome analyses.
• Spearhead efforts in identifying, developing, and implementing innovative quantitative protein mass spectrometry technologies for chemo-proteomics and general proteomics across the oncology portfolio.
• Participate in all aspects of laboratory operations including proteomic technology evaluation, proteomic software evaluation, development of quantitative sample preparation methods and peptide separation methods, data analysis and interpretation.
• Presenting and reporting data with follow up discussions for project teams including data impact and interpretation.
• Complete and effectively manage multiple projects within timelines ensuring that all tasks and responsibilities are carried out according to high scientific and ethical standards.
• Mentor and cross-train colleagues in protein mass spectrometry workflows.
• Build collaborative and strategic partnerships with internal and external research teams (leading universities and research institutes) to apply proteomics for oncology discovery, impacting all oncology research focuses including: Systems Therapeutics, Tumor Intrinsic Biology, Immuno-oncology and Epigenetics.
• Publications in peer-reviewed scientific journals and external/internal presentations are expected.
• Ph.D. in biological sciences, chemical biology, analytical chemistry, or a related field.
• 4 + years of post-doctoral or equivalent experience with applied mass spectrometry based proteomics required. Industry experience and oncology training is preferred.
• Experience and knowledge in the operation and maintenance of different types of mass spectrometers including Q Exactive Hybrid Quadrupole and Orbitrap Fusion Lumos Tribrid.
• Extensive knowledge and application of complex sample preparation methodologies as applied to protein mass spectrometry workflows.
• Ability to characterize protein post-translational modifications including phosphorylation, methylation, acetylation, PARylation and ubiquitination.
• Expertise in implementing quantitative mass spectrometry-based strategies including isotopic labeling, isobaric tagging, SILAC, and label-free analysis (DIA, PRM/SRM) methods.
• Applied knowledge with MS software analysis pipelines including IP2, Proteome Discoverer, MaxQuant and Skyline.
• General understanding of chemical proteomics methodologies including affinity enrichment, target occupancy and Chem-Seq.
• General understanding of target deconvolution methods including CETSA, DARTS, FUSION and chemical probes/proteomics.
• Excellent verbal and written communication skills.
• Relocation eligible
• Eligible for employee referral bonus
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Research and Development