Pfizer Senior Scientist, Validation & Phenotyping in Cambridge, Massachusetts
Pfizer's in vivo functional genomics unit is focused on rapid generation of transgenic mouse models for validation of new drug targets, mechanistic understanding of disease pathology and preclinical testing. The unit applies the latest technical developments in gene editing and transgenic technology to create First-In-Class genetically engineered mouse models (GEMMs).
The position will independently perform in vitro and in vivo validation of new models generated in-house and lead collaborative efforts for early phenotyping to facilitate target validation.
Develop and execute a strategy for early phenotyping utilizing resources available across the Pfizer sites in collaboration with Comparative Medicine and research units
Assimilate new analysis methods from different sources to generate high quality data
Analyze and present early phenotyping data to collaborators for fast decision making
Perform genome editing in zygotes and cultured cells to validate model design
Identify and characterize animals with a desired editing via genotyping and expression analyses
Test and implement the latest gene editing technologies for creation of animal models
Master's degree with >7 years of experience or Ph.D. in molecular and cell biology or related field
Hands-on experience with in vivo models of human diseases
Strong understanding of mouse genetics and practical steps involved in GEMM creation
Direct experience with routine molecular biology techniques and advanced gene expression analyses such as ddPCR and NGS
Experience in pathobiology, histology and imaging
Experience in cell engineering
Ability to engage closely in multiple projects and to track record of timely execution
Excellent interpersonal and communication skills; ability to maintain productive and congenial collaborations in a fast-paced environment
Post-doctoral experience or equivalent
Expertise in dissecting and analyzing diverse mouse tissues
Experience in mouse embryonic stem cell culture and gene targeting
Experience in CRISPR gene editing technologies
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Research and Development