Pfizer Senior Scientist, Protein Biochemistry & Purification in Cambridge, Massachusetts

Pfizer Biomedical Institute @ Kendall Square, Cambridge, has an open position at the Senior Scientist level for a highly motivated and talented Protein Biochemist in the Target Protein Engineering (TPE) Group within the Department of Biomedicine Design (BMD). The TPE mission is focused on enabling biotherapeutic drug discovery programs across all therapeutic research units by producing high quality target proteins, cell lines, and other tools for biotherapeutic antibody discovery and characterization, biological and biophysical assays, and protein structure.


  • Independently design and perform purification strategies for recombinant proteins from a variety of host cells (mammalian, insect, and bacterial cells).

  • Troubleshoot and persevere on difficult projects to successful conclusions.

  • Physical characterization of purified proteins using appropriate instrumentation.

  • Responsible for supporting the needs of multiple drug discovery projects and science objectives in parallel.

  • Close collaboration with members of the TPE group to advance the group's remit.

  • Maintain close interactions with colleagues across the BMD group, and liaise with cross-functional project teams to advise and to communicate progress.


  • Ph.D. degree in biochemistry, structural biology or a related discipline with at least 2 years relevant postdoctoral experience. BS or MS with 5+ years highly relevant experience will be considered.

  • Strong documented experience in protein biochemistry, protein purification (using affinity and traditional chromatographic techniques), protein modification using enzymatic and chemical methods, and biophysical characterization.

  • Hands-on, extensive experience with instrumentation essential for protein purification, especially AKTA FPLC and Agilent HPLC systems, SEC-MALS, mass spectrometer.

  • Demonstrated capabilities to independently design, implement, and troubleshoot difficult protein purification and protein modification experiments and persist to successful outcomes.

  • Experience in developing and performing refolding protocols for insoluble proteins from bacteria.

  • Publication record reflecting scientific drive and creativity in protein sciences.

  • Must be able to thrive and deliver results on goals in a fast-paced, dynamic and highly multi-tasking environment.

  • Must work well independently and as a member of teams in an inclusive manner.

  • Must possess excellent communication and presentation skills.


  • Experience in purification and handling of various classes of secreted proteins.

  • Experience with methods for successful purification of integral membrane proteins is highly preferred.

  • Experience with creating and characterizing protein-protein and/or protein-small molecule complexes.

  • Familiarity with recombinant expression of proteins in mammalian, insect and/or bacterial will be given special consideration.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Other Job Details:

  • Eligible for Relocation Package: yes

  • Eligible for Employee Referral Bonus: yes

Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.