Pfizer Senior Scientist, Antibody Discovery for Target Validation in Cambridge, Massachusetts

ROLE SUMMARY

BioMedicine Design's Endogenous Antibody Discovery Group is seeking a Senior Scientist to lead a team of scientists to design and drive antibody discovery campaigns. The antibodies will be tested in vitro and in vivo to validate targets to support Pfizer's early stage biologics discovery efforts. The candidate will be responsible for target evaluation in order to design optimal immunization strategies; screening of immune sera by ELISA and other methods in a high-throughput environment; generation and screening of hybridomas; and VH-VL molecular cloning of lead hybridoma candidates. The candidate will also learn and apply existing microfluidics-based single B cell screening methods in order to increase the number of selected antibodies and reduce timelines.

The successful candidate will have experience in some of the following areas:

  • Design of immunization strategies to elicit antibodies in rodents.Familiarity with dosing rodents.

  • Antibody discovery using hybridoma technology.Experience with primary B cells, including antibody isolation with in vitro methods, highly desirable.

  • High throughput screens such as protein ELISA, cell-based ELISA and flow cytometry.

  • Integrated tissue culture and screening automation.

ROLE RESPONSIBILITIES

  • Analysis of target protein structure and design of immunization strategies to elicit relevant immune titers

  • Determination of immune titers by ELISA and flow cytometry

  • Use of hybridoma technology and operation of integrated tissue culture automation system Implementation of established molecular cloning workflows Introduction of new methods identified to streamline and automate molecular cloning of antibodies

  • Optimizing transient expression of antibody clones

  • Preparation and delivery of detailed presentations, both written and verbal, to project teams

  • Attention to detail, effective communication and coordination of work in highly matrixed project team environment

BASIC QUALIFICATIONS

  • PhD with 0-2 years of experience in the pharmaceutical industry, Master's Degree with 3-5 years of experience in pharmaceutical industry or Bachelor's Degree with 6-8 years of experience in the pharmaceutical industry. Concentration of degree must be in Biochemistry, Biology, Immunology or related field.

  • Background in hybridoma generation with experience with immunizations of rodents critical.

  • Experience with VH-VL molecular cloning essential.

PREFERRED QUALIFICATIONS

  • Experience with antibody isolation from single B cells, high-throughput assay development and automation highly desirable.

  • Management of employees within a lab setting would be preferred.

Other Job Details:

  • Additional Location Details: Cambridge, MA

  • Eligible for Employee Referral Bonus: Yes

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.

Copyright © 2002-2011 Pfizer Inc. All rights reserved. This information is intended only for residents of the United States. The product information provided in this site is intended only for residents of the United States. The products discussed herein may have different product labeling in different countries. Pfizer Inc is a pharmaceutical company committed to helping people improve their health by discovering and developing medicines.
DirectEmployers